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    Pneumococcal Cell Biology

    How the oval shaped pneumococcus controls the initiation of DNA replication, segregates its chromosomes and divides at the right time at the right place remains elusive. We develop novel tools that allow for live cell imaging of the pneumococcus, such as codon optimised fast folding green and red fluorescent proteins. In addition, we continuously develop software and statistics tools to perform a quantitative analysis on cell biology (microscopy images) data.

    One puzzling and open question we are chasing down at the moment is why Z-ring formation occurs coincidentally with the initiation of DNA replication at midcell. See our recent observations in this field here: Kjos & Veening, MolMicro 2014, van Raaphorst et al., PNAS 2017.